Research

Cell nucleus, regulation of gene expression, nuleoskeleton, nuclear myosins and phospholipids, microscopy

In diploid mammalian cells, some 6×109 base pairs of DNA fold as a nucleoprotein complex (i.e. chromatin) into higher-order arrays so as to fit in a nucleus measuring only 10 µm. The nucleus also contains machineries for transcription of genes and processing of RNA products, and for precise DNA replication, repair and recombination. Nuclear interior is therefore functionally highly compartmentalized, and the recent evidence points strongly to structure-related regulation of nuclear functions – however, the mechanisms forming the 3D-structure of the nucleus are still mostly obscure. We therefore employ a multi-disciplinary approach in order to study nuclear functions in relation to the higher-order nuclear structures, e.g. nuclear bodies, the nucleolus, and the nucleoskeleton. Our research concentrates on: (1) the relationship between nuclear compartmentalization and regulation of gene expression, (2) structure, dynamics, and function of the nucleoskeleton, which contributes to the nuclear compartmentalization, (3) functions of nuclear myosins and actins in transcription and gene expression, (4) functions of nuclear lipids, (5) development of new microscopy methods.

Last change: February 23, 2016