Transgenic and Archiving Module

Facility leader
Radislav Sedláček
radislav.sedlacek@img.cas.cz
Director of Czech Centre for Phenogenomics - www.phenogenomics.cz

Location

CCP/BIOCEV, Průmyslová 595, 252 50 Vestec

Contact

Genome Engineering and Model Generation
Phone: +420 325 873 222

Breeding, Cryopreservation and EMMA/Infrafrontier Services
Phone: +420 325 873 256

E-mail: ccp-tam@img.cas.cz

About

The Transgenic and Archiving Module (TAM) is a key part of the Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, responsible for generation of novel genetically modified mice and rats using state-of-the-art technologies. TAM provides a complete service, from the initial gene-targeting design, generation of tools and transgenic rodent models to the genotyping and breeding of desired animal models.

Our services include zygote electroporation of CRISPR/Cas-based targeting tools, pronuclear microinjection of DNA constructs into mouse zygotes, microinjection of targeted ES cell lines into morulas (8-stage cell embryo) to produce chimeric mice; mouse archiving (cryopreservation of embryos and sperm); and recovery of live mice from cryopreserved embryos and sperm, analysis of sperm viability, rederivation of mouse strains and lines, and others. We also provide consultation and assistance services, and information on the design and use of genetically modified transgenic mice.

All of our services are also available to external institutions and researches irrespective whether they are from academic or profit organizations.

For more information visit the TAM website.