RNA Structure and Function – Faculty of Science, Charles University

The course „RNA Structure and Function“ (MB150P91E) is planned for master and PhD students and extends basic molecular and cell biology courses. The course is intended mainly for students who want to extend their knowledge about regulation of gene expression and about the ever-growing world of RNA. Individual lectures are focused on processing of mRNA as well as synthesis, processing and function of non-coding RNAs. The majority of discoveries about RNAs is documented by original scientific publications with explanation of key experiments.



1. Introduction

RNA polymerase I transcripts


ribosomal RNA synthesis and processing, nucleolar structure and function, ribosome structure, and function

4. RNA polymerase II transcripts
5. regulation of transcription through RNA network, connection between transcription and processing
6. snRNA and snoRNA biogenesis, scaRNAs. telomerase structure and function
7. pre-mRNA processing (capping, splicing, editing, polyA) , alternative splicing, mRNA localization and stability
8. cellular structures involved in RNA metabolism
9. RNA polymerase III transcripts
10. transcription, processing and function of tRNAs, 7SK, SRP, U6, RNA P and MRP
11. Catalytic RNAs
12. ribozymes and riboswitches, spliceosome and ribosome as ribozymes
13. Long non-coding RNAs
14. transcription, processing andfunction of HOTAIR, Air, MALAT1 and 2
15. RNAi
16. synthesis, processing and function of miRNA, piwiRNA, siRNA


Advanced Techniques in Fluorescence Microscopy

The major objective of the course is to introduce powerfull tools of basic and advanced fluorescence microscopy to potential users: PhD students and Postdocs with research interests in cell and developmental biology.

More information – http://course.img.cas.cz/atfm/



FRAP fitting FRAP_fitting
MATLAB scripts FRAP_fitting_scripts


Lectures RNA (password protected)

Poslední změna: 22. říjen 2019