Confocal Microscopes

Inverted microscope DMI6000 with confocal head Leica TCS SP5 AOBS Tandem is a confocal laser scanning microscope in which all filtering and beam-splitting functions are performed by acusto-optical components, which makes the system extremely flexible. The tandem scanner allows to switch between standard or very fast resonant scanner, which is useful especially for dynamic processes in live cells. For that reason, the microscope is enclosed in climate chamber for work at 37°C and regulated CO2 atmosphere. The system is equipped with both FRAP and FRET modules.

Inverted microscope DMi8 with confocal head Leica TCS SP8 is a confocal laser scanning microscope with full transmitted light equipment including transmitted light PMT and differential interference contrast (DIC) available for all objectives. Leica TCS SP8 it is a mirror-based system, using iterference filters for light filtering and beam-splitting. The laser power is modulated by acusto-optical tunable filter (AOTF).

Fully motorized inverted microscope DMi8 with confocal head Leica STELLARIS 8 equipped with wide-range while light laser with pulse picker (WLL PP) and highly sensitive time-resolved detection allows a variety of precise acquisition from standard confocal through very fast live cell acquisition up to large object imaging. The system is equipped with several acquisition modules for proceeding photo-kinetic experiments, FLIM or FLIM-FRET based experiments to the high resolution imaging followed with unique adaptive deconvolution module. The combination of AOBS and spectral sliders on detector cascade allows the fine tuning in selection of an excitation laser line and precise definition of emission spectra. For long-term live cell experiments are secured with hardware autofocus system (AFC). The system is equipped with incubation secures e.g. 37 °C and CO2 controlled chamber.

Dragonfly is a fully motorized multi-modal imaging platform with confocal and widefield modes. The heart of the microscope is the confocal spinning disk which in combination with high-speed sCMOS and high-sensitive EMCCD cameras provides the system with exceptional speed and sensitivity. The low phototoxicity and photobleaching is ideal for live or delicate specimens. Dragonfly is 10-20 times faster than a traditional laser scanning confocal, which makes it optimal for imaging of fast dynamic events in live cell or for high throughput experiments. Photomanipulation experiments can be performed with Mosaic, a tool that allows for simultaneous illumination of multiple regions of interest of flexible shape and size.
The semi-automated microinjector suited for adherent cells microinjection is also available.