Institute of Molecular Genetics of the Czech Academy of Sciences
Upright routine widefield fluorescence microscope with motorized stage, fully automated transmitted and fluorescence light axis, and both monochromatic and color cameras. The monochromatic one is a high resolution sensitive grayscale sCMOS camera. The LAS X Navigator tool allows to create high-resolution image tile-scans of large areas using the precise motorized stage.
| Methods | Routine fluorescence imaging True color RGB imaging in transmitted light mode Brightfield/Darkfield and Phase contrast Large tile-scans & auto merge in LAS X Navigator |
| Illumination | HLX 100 W Halogen lamp for transmitted light HXP 120W/45C VIS Hg lamp – Leica EL6000 for fluorescence |
| Objectives | HC PL FLUOTAR 5x/0.15 DRY; FWD 12 | BF, DF, POL HC PL APO 10x/0.40 DRY PH1; FWD 2.2; CG 0.17 | BF, DF, POL, PH HC PLAN APO 20x/0.70 DRY PH2; FWD 0.59; CG 0.17 | BF, DF, POL, PH HCX PL APO 40x/0.75 DRY PH2; CG 0.17 | BF, DF, POL, PH HCX PL APO 63x/1.40 OIL PH3 CS; FWD 0.1; CG 0.17 | BF, POL, PH HCX PL APO 100x/1.40-0.70 OIL; FWD 0.09; CG 0.17 | BF, DF, POL |
| Filtercubes | DAPI (A4) (Ex: 360/40; DM 400; Em: 470/40) FITC (L5) (Ex: 480/40; DM 505; Em: 527/30) TRITC (N3) (Ex: 546/12; DM 565; Em: 600/40) Cy5 (Y5)(Ex: 620/60; DM 660; Em: 700/75) |
| Cameras | Leica K3C – color CCD camera, 2,4 μm pixel Leica DFC 9000 – monochromatic sCMOS camera; 6,5 µm pixel, QE: min. 82 % |
| Stage | Motorized microscope stage xyz |
| Software | LAS X, 64 Bit |
| Location | room no. 0.172 (Green) |
| Phone | ext. 3172 |
| Booking | Calpendo (“DM6000” ) |
Upright widefield fluorescence microscope with motorized stage, fully automated transmitted and fluorescence light axis and high-resolution sensitive monochromatic 16-bit sCMOS camera. System enables image rotation by software controlled camera rotator.
| Methods | Routine fluorescence imaging Brightfield/Darkfield and Phase contrast |
| Illumination | HLX 100 W Halogen lamp for transmitted light HXP 120W/45C VIS Hg lamp – Leica EL6000 for fluorescence |
| Objectives | HC PL FLUOTAR 6.3x/0.13 DRY; FWD 12.860 | BF, POL HC PL FLUOTAR 10x/0.30 DRY PH1; FWD 11.0; CG 0.17 | BF, DF, POL, PH HC PL FLUOTAR 20x/0.40 DRY PH1; FWD 7.5; CG 0.17 | BF, DF, POL, PH HC PLAN APO 20x/0.70 DRY PH2; FWD 0.59; CG 0.17 | BF, DF, POL, PH HCX PL APO 40x/0.75 DRY PH2; FWD 0.28 CG 0.17 | BF, DF, POL, PH HCX PL APO 63x/1.40 OIL; FWD 0.14; CG 0.17 | BF, POL, PH HCX PL FLUOTAR 100x/1.3 OIL PH3; FWD 0.13; CG 0.17 | BF, POL |
| Filtercubes | DAPI (A) (Ex: 360/40; DM 400; Em: LP 425) GFP (Ex: 470/40; DM 495; Em: 525/50) TRITC (N3) (Ex: 546/12; DM 565; Em: 600/40) Cy5 (Y5)(Ex: 620/60; DM 660; Em: 700/75) |
| Cameras | Leica DFC 9000 – monochromatic sCMOS camera; 6,5 µm pixel, QE: min. 82 % |
| Stage | Motorized microscope stage xyz |
| Software | LAS X, 64 Bit |
| Additional equipment | Electronic Camera rotator allows 360° physical rotation |
| Location | room no. 0.172 (Green) |
| Phone | ext. 3172 |
| Booking | Calpendo (“DM6000-2”) |
Inverted widefield fluorescence microscope with motorized stage and fully automated transmitted and fluorescence light axis. The system is dedicated for live cell imaging. The stability of long-term time-lapse experiments with live cells is ensured by hardware autofocus system and environmental chamber for temperature, CO2 and humidity control. The microscope is also equipped with infinity port laser-scanner for photo-kinetic experiments (FRAP, iFRAP, Photo-activation). All the features are fully implemented in LAS X software. The LAS X Navigator tool allows to create high-resolution image tile-scans of large areas using the precise motorized stage.
| Methods | Routine fluorescence imaging True color RGB imaging in transmitted light mode Brightfield/Darkfield, Phase contrast, Modulation contrast Large tile-scans & auto merge in LAS X Navigator Time-lapse experiments with HW autofocus Multi-positions experiment Photo-kinetic experiments Large tile-scans & auto merge in LAS X Navigator |
| Illumination | LED light source for transmitted light HXP 120W/45C VIS Hg lamp – Leica EL6000 for fluorescence lasers for photomanipulation: – 405 nm laser (50 mW) – 488 nm laser (50 mW) |
| Objectives | HC PL FLUOTAR 5x/0.25, FWD 12 | BF, POL, PH, IMC N PLAN 10x/0.25 DRY; FWD 17.6 | BF, POL, PH, IMC HCX PL FLUOTAR L 20x/0.40 DRY CORR; FWD 6.9; CG 0-2 | BF, POL, PH, IMC HCX PL FLUOTAR 40x/0.95 DRY CORR; FWD 3.3; CG 0-2 | BF, POL, PH, IMC HCX PL APO 40x/1.25 oil | BF, POL HCX PL APO 63x/1.40-0.60 OIL; FWD 0.1; CG 0.17 | BF, POLBF, POL |
| Filtercubes | DAPI (A4) (Ex: 350/50; DM 400; Em: 460/50) FITC (Ex: 480/40; DM 505; Em: 527/30) TRITC (Ex: 546/10; DM 560; Em: 585/40) Y5 (Cy5) (Ex: 620/60; DM 660; Em: 700/75) |
| Cameras | Leica K3C – color CCD camera, 2,4 μm pixel Leica DFC 9000 – monochromatic sCMOS camera; 6,5 µm pixel, QE: min. 82 % |
| Stage | Motorized microscope stage xyz |
| Aditional equipment | PEACON incubation unit (CO2, temperature, humidity) |
| Software | LAS X |
| Location | room no. 0.171 (Orange) |
| Phone | ext. 3171 |
| Booking | Calpendo (“DMI 8” ) |
Inverted widefield fluorescence microscope DMi6000 with fully automated transmitted and fluorescence light axis. The transmitted light equipment includes differential interference contrast (DIC) prisms. An incubation chamber makes the system suitable for live-cell imaging. The total internal reflection fluorescence illumination module (Leica AM TIRF MC) controls the angle of the excitation beam and makes use of the total reflection to excite only molecules in the thin section in contact with the cover glass.
| Methods | Routine fluorescence imaging Brightfield, Nomarski contrast (DIC) Multi-positions experiment Time-lapse experiments TIRF microscopy experiments |
| Illumination | HLX 100 W Halogen lamp for transmitted light HXP 120W/45C VIS Hg lamp – Leica EL6000 for fluorescence solid-state TIRF laser 405 nm solid-state TIRF laser 488 nm solid-state TIRF laser 561 nm solid-state TIRF laser 633 nm |
| Objectives | HCX PL FLUOTAR 10x/0.30 DRY PH1; FWD 11.0 | BF, POL HCX PL FLUOTAR L 20x/0.40 CORR DRY PH1; FWD 6.9; CG 0-2 | BF, POL N PLAN L 40x/0.55 CORR DRY; FWD 3.3; CG 0-2 | BF, POL HCX PL APO 63x/1.3 GLYC CORR 37°C; FWD 0.28; CG 0.14-0.18 | BF, POL, DIC HCX PL APO 63x/1.40-0,60 OIL CS; FWD 0.1; CG 0.17 | BF, POL, DIC HCX PL APO 100x/1.46 OIL CORR CS; FWD 0.09; CG 0.1-0.22 | BF, POL, DIC |
| Filtercubes | A (Ex: 360/40; DM 400; Em: LP425) CFP-T (Ex: 422/44; DM 455; Em: 480/40) GFP-T (Ex: 490/20; DM500; Em: 525/50) YFP-T (Ex: 490/20, Em: 535/30) Cy3-T (Ex: 560/10, Em: 610/65) Cy5-T (Ex: 635/10, Em: 720/60) TRI (B/G/R) (ExFW: 420/30; 495/15; 570/20, Em: 457/22;530/20;628/28) FRET kostka pro CFP/YFP (ExFW: 436/12, 500/20; EmFW: 467/37, 545/45) QUAD-T (ExFW: 405/12, 490/20, 560/30,635/20; EmFW: 450/50, 525/36,600/32,705/72) |
| Cameras | Leica DFC350FX R2 – monochromatic CCD camera; 6,4 µm pixel Andor iXon 897 – high sensitivity EMCCD back-iluminated camera, 512×512 px, suitable for single molecule live cell imaging; 16 µm pixel |
| Stage | Motorized microscope stage with Super Z-galvo scanning insert for fast and precise Z movement |
| Aditional equipment | Incubation unit (CO2, temperature, humidity) Acousto-optical tunable filter (AOTF) – laser control |
| Software | LAS AF |
| Location | room no. 0.171 (Orange) |
| Phone | ext. 3171 |
| Booking | Calpendo (“TIRF”) |
The system is built on the inverted widefield Olympus microscope version IX-71. The system is equipped with the HW autofocus system and an incubation chamber, and is suitable for multi-location and time-lapse experiments. The lasers for photomanipulation enable photokinetic experiments (FRAP) and FRET. The sensitive camera makes the microscope suitable for live cell imaging and low fluorescence intensity applications.
| Methods | Routine fluorescence imaging Brightfield, Nomarski contrast (DIC) Tile-scans Multi-positions experiment Time-lapse experiments with HW autofocus Photo-kinetic experiments |
| Illumination | Lumencore Spectra X LED light source for fluorescence lamp for transmitted light Lasers for photomanipulation (adjustable spot size): – 405 nm laser PoinSource iFlex2000 – 488 nm laser Coherent Sapphire (20 mW) |
| Objectives | U PLAN FL 20x/0.50 DRY PH1; FWD 1.6; CG 0.17 | BF, POL U PLAN FL 40x/0.75 DRY; FWD 0.51; CG 0.17 | BF, POL U APO/340 40x/1.35-0.65 CORR OIL; FWD 0.1; CG 0.17 | BF, POL PLAN APO N 60x/1.42 OIL; FWD 0.15; CG 0.17 | BF, POL, DIC |
| Exciation LED source | DAPI 390/18 CFP 438/24 GFP/FITC 475/28 YFP 513/17 RD-TR-PE 542/27 mCherry/Alexa594 575/25 Cy5 632/22 |
| Dichroic mirrors | Standard (DAPI, FITC, RD-TR-PE, Cy5) Live Cell (CFP, YFP, mCherry) Alexa (GFP, mCherry, Alexa 594) |
| Emission filter wheel | DAPI (435/48) FITC (523/36) RD-TR-PE (594/45) Alexa 594 (632/60) Cy5 (676/34) GFP (525/50) mCherry (632/60) CFP (470/24) YFP (559/38) |
| Eyepiece filers | The same as on emission filter wheel (excluding Cy5 filter) |
| Cameras | Photometrics CoolSANP HQ – high sensitive CCD camera, 1024×1024 px; 6,45 µm pixel |
| Stage | Motorized stage with xyz movement equipped with HW AF |
| Aditional equipment | Incubation unit (CO2, temperature, humidity) Antivibration table Hardware Ultimate autofocus for maintenance in-focus stability within the entire specimen |
| Software | SoftWorx – execution and evaluation 3D imaging – photo-kinetics experiments – time-lapse – FRET experiments – evaluation of co-localization – deconvolution |
| Location | room no. 0.171 (Orange) |
| Phone | ext. 3171 |
| Booking | Calpendo (“DVcore”) |
Zeiss Axiom Zoom.V16 – Fully motorized fluorescence macroscope with high resolution objectives is equipped with two cameras and a motorized 16:1 zoom with apochromatic correction. The system is well suited for a wide range of bigger samples and has superior brightness in large object fields. ApoTome.2 uses structured illumination to improve contrast and to increase resolution of the optical sections.
| Methods | Fluorescence imaging on widefield OR Apotome (optical sectioning) mode Transmitted and/or reflected light True color RGB imaging in transmitted light mode Brightfield/Darkfield, Opaque contrast Large tile-scans & auto merge in ZEN Blue software Time-lapse experiments Multi-positions experiment |
| Illumination | HXP 120 V – metal halid lamp for fluorescence CL 9000 LED CAN – transmitted light source CL 9000 LED CAN – incident light source |
| Objectives | PlanNeoFluar Z 1.0x/0.25; FWD 56 | BF, DF, oblique illumination + Reflected light PlanNeoFluar Z 2.3x/0.57; FWD 10.6 | BF, DF, oblique illumination + Reflected light |
| Fluorescence filtercubes | DAPI Filter set 49 (Ex: G 365; BS FT 395; Em: BP 445/50) eGFP Filter set 38 HE (Ex: BP 470/40; BS FT 495; Em: BP 525/50) Cy3 Filter set 63 HE (Ex: BP 572/25; BS FT 590; Em: BP 629/62) Cy5 Filter set 50 (Ex: BP 640/30; BS FT 660; Em: BP 690/50) |
| Cameras | ZEISS Axiocam 305 – color CMOS camera; 3,45 µm pixel ZEISS Axiocam 512 mono – monochromatic CCD camera; 3,1 µm pixel |
| Additional equipment | ApoTome.2 for optical sectioning |
| Software | ZEN Blue 2.3 |
| Location | room no. 0.175 (Blue) |
| Phone | ext. 2750 |
| Booking | Calpendo (“Apotome”) |