The course is intended for researchers who wish to become acquainted with the genome browser Ensembl and acquire theoretical background for CRISPR–based genome editing.
The course is intended for researchers who wish to become acquainted with the genome browser Ensembl and acquire theoretical background for CRISPR–based genome editing. Participants will become familiar with guidelines for devising optimal gene-targeting strategy, selection of gRNA and DNA template design.
Integral part of the course will be theoretical background and practical demonstration of state-of-the art delivery methods into mouse zygotes. Participants will have an opportunity to learn the revolutionary method iGonad directly from its inventors. In principle, successful participants should be able to become familiar with all relevant steps in the process of mutant model generation starting from its design to the production of gene-edited animals.
Each participant will have a chance to discuss his/her own individual gene-targeting project with top experts in the field. Moreover, a few individual projects will be selected for production of mutant animals by CCP team (only the material costs will be charged).
The course is divided into two individual modules:
Participation in an individual module is possible; however, we encourage participants to attend both modules to get an in-depth insight into the problematics.
The course has been designed for participants with an intermediate knowledge of molecular biology.
More information and registration – www.phenogenomics.cz.