Localization of programmed DNA breaks in the rat germ cells

  • published: 3. 5. 2021

An international team lead by Zdenek Trachtulec deciphered the control of the positioning of DNA breaks in the developing germ cells of laboratory rat. These DNA breaks are important for fertility. Nevertheless, inactivation of the Prdm9 gene, which controls the positioning of these breaks, does not always lead to sterility.

Delayed sperm development in Prdm9-deficient rats compared to controls. A, B: Immunohistochemistry of 21-days-old testes. Tubules were scored for the presence of cells expressing the PIWIL1 protein (154 to 368 tubules per animal, each animal depicted as a single dot in the plot). A, Plot (author: T. Kobets). B, Representative images: left, control; right, Prdm9-deficient male; photo: O. Mihola).

Picture – Delayed sperm development in Prdm9-deficient rats compared to controls.
A, B: Immunohistochemistry of 21-days-old testes. Tubules were scored for the presence of cells expressing the PIWIL1 protein (154 to 368 tubules per animal, each animal depicted as a single dot in the plot). A, Plot (author: T. Kobets). B, Representative images: left, control; right, Prdm9-deficient male; photo: O. Mihola).

 

Contact:

Zdeněk Trachtulec, Ph.D.,
phone: (+420) 325 873 109, e-mail: zdenek.trachtulec@img.cas.cz,
web: www.img.cas.cz/research/zdenek-trachtulec/

 

More information in publication – https://bmcbiol.biomedcentral.com.