Autonomous Microscopy: LAS X Navigator Expert

  • Date & Time: 6. 2. 2024 - 8. 2. 2024  |  9:00 - 17:00
  • Location: IMG building

Autonomous microscopy is an approach that changes the way how researchers explore biological samples. The meeting is oriented towards a comprehensive exploration of autonomous microscopy in the Leica LAS X Navigator Expert module.

This three-day workshop, held on the Leica Stellaris 8 confocal microscope, is an immersive, hands-on experience led by a Leica application specialist. Participants will have the opportunity to learn practical applications of automated detection of Regions of Interest (ROIs) and rare events (REs) using advanced microscopy techniques.

Admission is free of charge.

Event Overview

Day 1: Participants will be introduced to the theoretical aspects of autonomous microscopy in the morning, followed by a hands-on practical session in the afternoon.

Days 2: The workshop continues with hands-on practical sessions with mid-day lectures about the Stellaris 8 hardware.

Days 3: Hands-on practical sessions.


DAY 1 | THEORETICAL PART (room 0.195)
February 6th, 2024

09:45 – 10:00Invitation
10:00 – 10:30Leica
Introduction to LAS X Navigator and its implementation to Stellaris 8
10:30 – 10:50Jakub Ohnhajzer (IMG)
Routine with immunohisto slides
Coffee break
11:00 – 11:30Ivan Novotný (IMG)
When we need a confocal for our experiments
11:30 – 12:00Ondřej Černý (IMB)
Live bacteria, time-lapse and choice between tracking and bleaching
13:00 – 13:20Martin Anger (IAPG)
What scientist expect from the software in live-cell time-lapse experiment
13:20 – 13:45Sponsor presentation (Specion)
Leica TauSTED
13:45 – 14:15Leica
Autonomous Microscopy and Navigator Expert
14:15 – 14:35Marcela Filipová (IMC)
Confocal imaging and quantification of fluorescently-labeled nanoprobes internalized by cancer cells using Leica LAS X Navigator
Coffee break
14:50 – 15:20Leica
The Near Future of Microscopy and its Development
15:20 – 16:00Final broad discussion

DAY 2 and 3 | PRACTICAL PART (room 0.175)
February 7-8th, 2024

09:00 – 10:30
10:30 – 12:00
Slots on Stellaris 8 LAS X Navigator Expert, assisted with Leica application specialist.
12:00 – 13:00Lunch
13:00 – 14:30
14:30 – 16:00*
Slots on Stellaris 8 LAS X Navigator Expert, assisted with Leica application specialist.
* This slot is not available on Thursday, February 8th.



Ivan Novotný, Ph.D.

Light Microscopy Core Facility
Institute of Molecular Genetics of the Czech Academy of Sciences

Dr. Martin Kopecký