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Institute of Molecular Genetics of the Czech Academy of Sciences
Institute of Molecular Genetics of the Czech Academy of Sciences

Histology Laboratory

LocationIMG – building F, basement, room 01.156
Phone+420 29644 3194 (laboratory)

About

Histological laboratory provides equipment for tissue dehydratation, creation of paraffin blocks, tissue sectioning (paraffin, cryo, fresh tissue), deparaffination, H&E stain, antigen retrieval.

The facility is based on semi-self-service – tissue dehydratation is collective and handled by the staff, all the other steps are carried out by each user individually.

To prepare histological samples follow the Instructions.

People

OperationJakub Onhajzer, M.Sc.
Supervision Matyáš Šíma, Ph.D.

Reservation

  • To register for tissue processing, please fill up the reservation form. Your request will appear in the table below within 5 minutes.
  • Please note that reservation for creation of paraffin blocks after tissue dehydratation and for micro-sectioning is also required. For reservation, please use the IMG booking system Calpendo.

Open the list of reservations in a new tab

Explanation
first day = first day when you could have the samples ready for processing
last day = last day when you need to have the samples processed
expected processing = will be entered by the instrument operator

Gallery

Střevní sekreční buňky (hnědé), buněčná jádra kontrastovaná hematoxylinem (modrofialová)
Immunohistochemistry of intestinal secretory cells (brown), cell nuclei contrastained with Hematoxylin (blue-purple)
Intestinal tumor, Dapi (Blue; nuclei), ID4 (Green; secretory cells) and Trop2 (Red; tumor cells )
Intestinal tumor, Dapi (Blue; nuclei), ID4 (Green; secretory cells) and Trop2 (Red; tumor cells)
Játra - hepatocyty kolem portální žíly v jaterním parenchymu, Hoechst (modrá; jádra), Desmin (zelená; jaterní Itovy buňky) a E-Cadherin (červená; hepatocyty kolem portální žíly v jaterním parenchymu)
Liver – hepatocytes around portal vein in the liver parenchyma, Hoechst (Blue; nuclei), Desmin (Green; hepatic stellate cells) and E-Cadherin (Red; hepatocytes around portal vein in the liver parenchyma)
Kožní štěpy myši porostlé dermatofitními houbami (Hematoxylin a eosin barvení)
Mouse skin grafts covered with dermatophyte fungi (Hematoxylin and eosin staining)
Myší plíce s nádorem, Dapi (modrá; jádro), EpCam (zelená; epitelové buňky) a PCNA (červená; proliferující buňky)
Murine lungs with tumor, Dapi (Blue; Nuclei), EpCam (Green; Epithelial cells) and PCNA (Red; Proliferating cells)
Lendrum straining of the mouse intestine
Lendrum straining of the mouse intestine
β-catenin immunohistochemistry staining of the mouse intestine
β-catenin immunohistochemistry staining of the mouse intestine
Nuclear Fast Red staining of the mouse intestine
Nuclear Fast Red staining of the mouse intestine
lacZ staining of the transgenic mouse hair follicles contrastained with Nuclear Fast Red
lacZ staining of the transgenic mouse hair follicles contrastained with Nuclear Fast Red

Additional information about group

Home Equipment Instructions Terms and Conditions External User Access Links and Tips

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