Laboratory of Integrative Biology

We study the functional consequences of cytoskeleton organization in cell and tissue mechanics and mechanotransduction

+ mechanobiology, + cytoskeleton, cytolinkers, + cell junctions, + simple epithelia

Scientific Career – Milestones

since 2015: Research Group Leader, IMG, Prague
2003-2010: Research Fellow, Max Perutz Labs, University of Vienna, Austria
2002: PhD, Faculty of Science, Charles University, Prague

Plectin integrates cortical actin and keratin filament networks in epithelial sheets to provide support to intercellular junctions

By combining the CRISPR/Cas9-based gene editing approach and pharmacological inhibition with the novel cytoskeletal inhibitor plecstatin, we provided the first-ever evidence that plectin – in an F-actin dependent manner – orchestrates epithelial keratin filaments into a ‘rim-and-spoke’ configuration. [pubmed] [doi]

Role of plectin in biliary tree architecture and its stability in cholestasis

We have created a mouse model recapitulating the symptoms of epidermolysis bullosa in the liver. We have shown that epidermolysis-associated genes play a key role in liver adaptation to cholestasis. The study also highlighted the risks in patients with epidermolysis in combination with impaired bile production and excretion. [pubmed] [doi]

Plectin safeguards against intestinal inflammation by maintaining the functional organization and stability of intestinal epithelium

We have found that mice with plectin ablation in intestinal epithelial cells spontaneously developed a colitic phenotype – that is extensive detachment of intestinal epithelial cells from the basal membrane, ulceration, and mucosal inflammation. According to our study, antibiotic treatment and a low-residue liquid diet, which reduces mechanical stress, ameliorate intestinal epithelial damage. [pubmed] [doi]

Research Reports

2020-2022 / 2017-2019 / 2015-2016

Additional information about group