Laboratory of Biology of the Cell Nucleus

Equipment & Booking

Main techniques used in the laboratory

Techniques involve primarily: cell cultures, cell cycle synchronization, replication and transcription essays, RNAi, gene knock-outs, immunolabelling coupled with light (fluorescent, confocal) and advanced electron microscopy, protein immunoprecipitation and purification from cellular extracts, 2D electrophoresis, Western blotting, microinjections into living cells, molecular cloning, GFP technology, cell transfections, DNA/RNA hybridization in situ/on blots, production of antibodies, digital imaging, computer-assisted 3D reconstructions, image processing, stereology, production of monoclonal antibodies, knock-out mice.