Institute of Molecular Genetics of the Czech Academy of Sciences
Laboratory of Biology of the Cell Nucleus
Equipment & Booking
Main techniques used in the laboratory
Techniques involve primarily: cell cultures, cell cycle synchronization, replication and transcription essays, RNAi, gene knock-outs, immunolabelling coupled with light (fluorescent, confocal) and advanced electron microscopy, protein immunoprecipitation and purification from cellular extracts, 2D electrophoresis, Western blotting, microinjections into living cells, molecular cloning, GFP technology, cell transfections, DNA/RNA hybridization in situ/on blots, production of antibodies, digital imaging, computer-assisted 3D reconstructions, image processing, stereology, production of monoclonal antibodies, knock-out mice.