Institute of Molecular Genetics of the Czech Academy of Sciences
Laboratory of Epigenetic Regulations
Open PhD positions
Project: Evolution of small RNA silencing pathways in animals
Supervisor
Petr Svoboda
Project description
The lab currently studies evolution of mammalian RNA silencing pathways and focuses on several specific aspects of RNAi and piRNA pathways. A PhD candidate will have an opportunity to choose as a main project either evolution of small RNA biogenesis and effector complex formation or adaptations and functional divergence of the piRNA pathway. The laboratory uses rodents and slugs as main experimental models. Laboratory work will range from biochemistry of ribonucleoprotein complexes to genetic modifications and phenotype analysis (largely involving long and small RNA analyses by RNA sequencing).
Candidate profile
We are looking for a curious and motivated PhD student with some experience with molecular biology, experience with laboratory animals, mammalian cell culture, molecular cloning, protein biochemistry or bioinformatics will be an advantage.
Suggested reading
Ketting RF. The many faces of RNAi. Dev Cell. 2011 Feb 15;20(2):148-61. [pubmed] [doi]
Loubalova Z, Fulka H, Horvat F, Pasulka J, Malik R, Hirose M, Ogura A, Svoboda P: Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs. Nat Cell Biol 2021 23(9): 992-1001. [pubmed] [doi]
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O’Carroll D, Stefl R, Svoboda P: Structural and functional basis of mammalian microRNA biogenesis by Dicer. Mol Cell 2022 82(21): 4064-4079.e13. [pubmed] [doi]
