Institute of Molecular Genetics of the Czech Academy of Sciences
Laboratory of Cell Motility
Open PhD positions
Project: Microtubule arrangement in primary cilium axoneme
Primary cilia have signalling and sensory functions and serve as antennas in many human cell types. Their malfunctioning causes severe diseases called ciliopathies. The project will focus on understanding of the recently described arrangement of microtubules forming the primary cilium axoneme. These terminate at various distances from the ciliary base with only several extending to the very tip of the cilium, which is very different to the canonical axoneme of motile cilia. The project will combine advanced imaging approaches, such as high resolution live cell imaging, expansion microscopy, correlative light and electron microscopy, with micromanipulations and molecular biology techniques to study how is the microtubule arrangement established, how dynamic it is, what are its implications for ciliary processes, and whether its alteration leads to ciliary malfunctioning. The project is supported by a collaborative Czech-USA grant.
The candidate should have an interest in cell biology, in particular biology of the eukaryotic cytoskeleton. An experience in light or electron microscopy imaging and cell culturing would be beneficial. A good command of English is expected given the international nature of the project.
Kiesel P, Alvarez Viar G, Tsoy N, Maraspini R, Gorilak P, Varga V, Honigmann A, Pigino G: The molecular structure of mammalian primary cilia revealed by cryo-electron tomography. Nat Struct Mol Biol 2020. [pubmed] [doi]
Sun S, Fisher RL, Bowser SS, Pentecost BT, Sui H. Three-dimensional architecture of epithelial primary cilia. Proc Natl Acad Sci U S A 2019 116(19):9370-9379. [pubmed] [doi]