Project: Proteasome control of apoptosis regulation in cancer
Supervisor
Lukáš Čermák
Project description
This project aims to investigate the pivotal role of the proteasome in the regulation of apoptosis within cancer cells. The proteasome, a cellular complex responsible for protein degradation, is known to play a crucial role in maintaining cellular homeostasis. However, its specific involvement in modulating apoptosis in cancer cells remains understudied. Through a series of experiments utilizing cancer cell lines and proteasome inhibitors, we intend to unravel the intricate mechanisms by which the proteasome influences apoptotic pathways. By identifying key protein targets and understanding the interplay between the proteasome and apoptosis regulators, we hope to shed light on potential therapeutic strategies for manipulating apoptosis in cancer cells. This research not only contributes to our fundamental understanding of cell biology but also holds promise for the development of targeted therapies that exploit the proteasome’s control over apoptosis in the context of cancer treatment.
Candidate profile
We invite enthusiastic and goal-oriented students with a keen interest in molecular biology and basic understanding of biochemical methods to join this research project. A comprehensive understanding of fundamental molecular biology techniques will be highly beneficial in unraveling the intricacies of proteasome-mediated control of apoptosis in cancer cells.
Suggested reading
Shanshan Duan 1, Lukas Cermak, Julia K Pagan, Mario Rossi, Cinzia Martinengo, Paola Francia di Celle, Bjoern Chapuy, Margaret Shipp, Roberto Chiarle, Michele Pagano: FBXO11 targets BCL6 for degradation and is inactivated in diffuse large B-cell lymphomas. Nature 2012 481(7379): 90–93. [pubmed] [doi]
Dibus N, Korinek V, Cermak L: FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability. Front Cell Dev Biol 2022 10: 929288. [pubmed] [doi]
Dibus N, Zobalova E, Monleon MAM, Korinek V, Filipp D, Petrusova J, Sedlacek R, Kasparek P, Cermak L: FBXO38 Ubiquitin Ligase Controls Sertoli Cell Maturation. Front Cell Dev Biol 2022 10: 914053. [pubmed] [doi]
